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ORIGINAL ARTICLE
Year : 2012  |  Volume : 8  |  Issue : 29  |  Page : 60-64

Cytotoxicity of fucosterol containing fraction of marine algae against breast and colon carcinoma cell line


1 Department of Pharmacognosy and Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran 14155-6451, Iran
2 Khorramshahr University of Marine Science and Technology, Khuzestan, Iran
3 Department of Toxicology and Pharmacology and Pharmaceutical Science Research Center, Tehran University of Medical Sciences, Tehran 14155-6451, Iran

Correspondence Address:
Seyed Nasser Ostad
Department of Toxicology and Pharmacology and Pharmaceutical Science Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14155-6451
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.93327

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Context: Marine algae produce different secondary metabolites with a wide range of biological activities. Many studies have been achieved on the screening of biological effects of marine organisms and a lot of active compounds were isolated and characterized. Aims: In an attempt to find cytotoxic compound of hexane fraction, isolation, identification, and cytotoxicity of active compound of this fraction were performed. Materials and Methods: In this study, total methanolic (70%) extract and partition fractions of hexane, chloroform (CHCl 3 ), ethyl acetate (EtOAc), and MeOH-H 2 O of Sargassum angustifolium, Chondria dasyphylla, and Ulva flexuosa, collected from coastlines of the Persian Gulf in south of Iran, were studied against colon carcinoma (HT-29), colorectal adenocarcinoma (Caco-2), breast ductal carcinoma (T47D), and Swiss mouse embryo fibroblast (NIH 3T3) cell lines by MTT assay. Statistical Analysis Used: IC 50 (median growth inhibitory concentration) values were calculated by Sigmaplot (10) software. Results: Hexane fraction of Chondria dasyphylla (IC 50 82.26 ± 4.09 μg/ml) and MeOH-H 2 O fraction of Ulva flexuosa (IC 50 116.92 ± 8.58 μg/ml) showed cytotoxic activity against proliferation of T47D cells. Hexane fraction of Sargassum angustifolium was also observed for cytotoxicity against T47D and HT-29 cell lines (IC 50 166.42 ± 26.7 and 190.24 ± 52.8 μg/ml), respectively. An investigation of a component from the hexane fraction of Sargassum angustifolium yielded a steroidal metabolite, fucosterol, with cytotoxicity in T47D and HT29 (IC 50 27.94 ± 9.3 and 70.41 ± 7.5 μg/ml). Conclusions: These results indicated that fucosterol, the most abundant phytosterol in brown algae, is responsible for cytotoxic effect of this extract against breast and colon carcinoma cell lines.


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