|Year : 2011 | Volume
| Issue : 28 | Page : 334-337
Enhancement of the antibiotic activity of erythromycin by volatile compounds of Lippia alba (Mill.) N.E. Brown against Staphylococcus aureus
Helenicy N.H. Veras, Adriana R Campos, Fabíola F.G. Rodrigues, Marco A Botelho, Henrique D.M. Coutinho, Irwin R.A. Menezes, José Galberto M da Costa
Programa de Pós-Graduação em Bioprospecção Molecular - Laboratório de Pesquisas de Produtos Naturais - Universidade Regional do Cariri - CEP 63105-000 - Pimenta - Crato - Ceará, Brazil
|Date of Submission||07-Mar-2011|
|Date of Decision||14-Mar-2011|
|Date of Web Publication||30-Nov-2011|
José Galberto M da Costa
Programa de Pós-Graduação em Bioprospecção Molecular, Departamento de Química Biológica, Laboratório de Pesquisa de Produtos Naturais, Universidade Regional do Cariri, Rua Cel. Antônio Luiz 1161, 63105-000 Crato-CE
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Lippia alba (Mill.) N.E. Brown, popularly known as "erva-cidreira," is commonly found in northeastern Brazil. The leaves tea is used to treat digestive disturbances, nausea, cough, and bronchitis. Objective: This work reports the chemical composition and erythromycin-modifying activity by gaseous contact against Staphylococcus aureus. Materials and Methods: The leaves of L. alba were subjected to hydrodistillation, and the essential oil extracted was examined with respect to the chemical composition, by gas chromatography-mass spectrometry (GC-MS), and the essential oil extracted was evaluated for antibacterial and antibiotic-modifying activity by gaseous contact. Results: The overall yield of essential oil obtained by hydrodistillation was 0.52%. The GC-MS analysis has led to the identification of the main components: geranial (31.4%) and neral (29.5%). It was verified that the essential oil interfered with erythromycin antibiotic activity against S. aureus ATCC 25923 was enhanced (221.4%) in the presence of 12% essential oil. The 3% essential oil increased the effect against S. aureus ATCC 25923 (41.6%) and S. aureus ATCC 6538 (58.3%). Conclusion: The essential oil of L. alba influences the activity of erythromycin and may be used as an adjuvant in antibiotic therapy against respiratory tract bacterial pathogens.
Keywords: Chemical composition, erythromycin, essential oil, Lippia alba, modulatory activity
|How to cite this article:|
Veras HN, Campos AR, Rodrigues FF, Botelho MA, Coutinho HD, Menezes IR, da Costa JM. Enhancement of the antibiotic activity of erythromycin by volatile compounds of Lippia alba (Mill.) N.E. Brown against Staphylococcus aureus. Phcog Mag 2011;7:334-7
|How to cite this URL:|
Veras HN, Campos AR, Rodrigues FF, Botelho MA, Coutinho HD, Menezes IR, da Costa JM. Enhancement of the antibiotic activity of erythromycin by volatile compounds of Lippia alba (Mill.) N.E. Brown against Staphylococcus aureus. Phcog Mag [serial online] 2011 [cited 2019 Jun 20];7:334-7. Available from: http://www.phcog.com/text.asp?2011/7/28/334/90415
| Introduction|| |
Knowledge on medicinal plants sometimes means the only therapeutic resource of some communities and ethnic groups.  Although the antibacterial properties of essential oils have been long recognized, the recent interest in alternative naturally derived antimicrobials has led to a renewed scientific interest in these substances. 
The genus Lippia (Verbenaceae) comprises about 200 species of herbs, shrubs, and small trees.  Lippia alba (Mill.) N.E. Brown, popularly known as "erva-cidreira," is commonly found in northeast Brazil. In many cases, leaves and flowers infusions or decoctions are employed orally to treat digestive disturbances, nausea, cough, and bronchitis. ,
Erythromycin belongs to the class of drugs known as macrolides and it acts on the ribosomal 50S subunit inhibiting the protein synthesis.  It is estimated that 15% of Staphylococcus aureus strains are resistant to erythromycin and this drug is not recommended for empiric treatment of staphylococcal skin and soft tissues infections. A relatively high incidence of nausea and vomiting (oral formulation) or phlebitis (endovenous formulation) is reported.  Recent studies  showed that S. aureus is the most frequent microorganism in nasopharynx of children and 14% of the isolates were no susceptible to erythromycin.
This work reports the modulatory antimicrobial activity of the Lippia alba (Mill.) N. E. Brown essential volatile components by the gaseous contact method.
| Materials and Methods|| |
Leaves of Lippia alba (Mill.) N.E. Brown were collected in March, 2010, from the Small Aromatic and Medicinal Plants Garden of the Natural Products Research Laboratory (LPPN) at University Regional do Cariri (URCA), Crato of county, Cearα state, Brazil. A voucher specimen was sent to the Herbarium Caririense Dαrdano de Andrade - Lima - HCDAL, Department of Biological Sciences (URCA), which is deposited on the registration n° 5059.
Obtention of the essential oil
Samples of fresh leaves (236 g) were triturated and submitted to hydrodistillation process, in a Clevenger-type apparatus for 2 hours, resulting in essential oil yield of 0.52%. The collected essential oil was subsequently dried by anhydrous sodium sulfate (Na 2 SO 4 ), and stored under refrigeration at <4°C until be tested.
Analysis of the essential oil
Analysis by CG/MS of the essential oil was carried out on a Hewlett-Packard Model 5971 GC/MS using a nonpolar DB-1-fused silica capillary column (30 m x 0.25 mm i.d., 0.25 m film thickness); carrier gas helium, flow rate 0.8 ml/min and with split mode. The injector temperature and detector temperature were 250°C and 200°C, respectively. The column temperature was programmed from 35°C to 180°C at 4°C/min and then 180°C to 250°C at 10°C/min. Mass spectra were recorded from 30 to 450 m/z. Individual components were identified by matching their 70 eV mass spectra with those of the spectrometer database using the Wiley L-built library and two other computer libraries MS searches using retention indices as a preselection routine,  as well as by visual comparison of the fragmentation pattern with those reported in the literature. 
Modulatory activity by gaseous contact
Antibacterial activity of the essential oil from L. alba (EOLA) was assayed using gaseous contact. An amount of 50 μg of oil was dissolved in 50 μl of DMSO (1 : 1). A two-fold dilution series of this essential oil solution was prepared: 50, 25, 12, 6, and 3 μg/ml of oil.
Different S. aureus strains-ATCC 12692, ATCC 25923, and ATCC 6538-were used as standard. Brain heart infusion broth was used for bacterial growth (24 hours, 37°C). 10 5 CFU/ml of the inoculum from overnight culture of each bacterial species were inoculated in Mueller Hinton Petri dish More Detailses (90 mm diameter) by streaking method. Disks containing erythromycin (15 μg, LABORCLIN® ) were used to determine changes in diameter of the zone of inhibition of studied strains.
After this, 50 μl of each dilution was added to the upper Petri dish and incubated for 24 hours at room temperature. Petri dishes without the essential oil and with DMSO were used as positive and negative controls, respectively.
| Results and Discussion|| |
As can be observed in [Table 1], geranial (31.5%) and neral (29.5%) were identified as the main components of L. alba essential oil. This chemical composition profile indicates that the natural product presents antimicrobial activity. According to Schuck et al.,  citral has been shown to be more efficient than nystatin and benzalkonium chloride against Candida albicans strains. As stated by Onawunmi,  alkaline pH increased citral activity and it can be used combined with others agents to enhance their antimicrobial activity.
As shown in [Table 2], the synergic effect was verified for all strains tested. The most expressive effect was the 221.4% increase of the diameter of the zone of inhibition to erythromycin on S. aureus ATCC 25923 in the presence of 12% EOLA. 3% EOLA increased the erythromycin activity against S. aureus ATCC 25923 and ATCC 6538 (12.5% and 35.7%, respectively).
|Table 2: Modification of the antibiotic activity of the volatile constituents of L. alba essential oil by gaseous contact on S. aureus ATCC 12692, ATCC 25923, ATCC 6538|
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The antimicrobial activity of L. alba extracts and essential oil has been described in some studies; ,, however, there is no report about the synergic effect between the essential oil and antibiotics by the gaseous contact method.
Essential oils may interact with and affect the plasma membrane, interfering with respiratory chain activity and energy production.  Besides this, the lipophilic structure of plasma membrane facilitates the antibiotic entrance into the bacteria cell and a consequent increase in its activity. , Impairment of bacterial enzyme systems may also be a potential mechanism of action due to the combination of direct vapor absorption by the microorganisms and vapor absorption through the medium. 
Several studies have reported the antimicrobial activity of essential oils and plant extracts. The essential oil of the Mentha piperita was effective against Gram-positive and Gram-negative strains, and antioxidant activity.  Tannins, alkaloids, and flavonoids in the ethanol extracts from Lantana montevidensis, Zanthoxylum rhoifolium, and Croton zenhtneri were responsible for the antimicrobial activity demonstrated.  These and other studies confirm the popular use of natural products from medicinal plants to combat several diseases.
Most of Lippia species are used to treat respiratory affections. In Central and South America (Guatemala, Venezuela, Brazil), L. alba is also employed as a remedy for colds, grippe, bronchitis, coughs, and asthma.  Essential oils, such as eucalyptus (Eucalyptus globulus Labill), act on the respiratory system, and possess, by oral and inhalatory ways, expectorant, fluidizing, and antiseptic activities of bronchial secretion. 
It is known that it is very common to use plants as self-medication supply concomitant to allopathic drugs (55.9%).  Another study  demonstrate that 41.7% of people have associated medicinal plants with some drug, before searching for a medical service. Because of this, it is necessary to improve the studies about the possible interactions between natural products and allopathic drugs.
Plant-derived natural products, in some cases, can interfere with the effectiveness of antibiotics being used for the clinical treatment. ,,,, The present article demonstrates that the volatile components of EOLA, in association with erythromycin, are able to promote the enhancement of the antibiotic activity.
The results are relevant and promising, considering the synergic association between L. alba essential oil and erythromycin as a possible therapeutic alternative to treat respiratory infections. So, new researches must be propounded in order to elucidate the possible action mechanisms involved.
| References|| |
|1.||Di Stasi LC. Plantas medicinais: Arte e ciência: Um guia de estudo Interdisciplinar. São Paulo: UNESP; 1996. |
|2.||Gutierrez J, Barry-Ryan C, Bourke P. The antimicrobial efficacy of plant essential oil combinations and interactions with food ingredients. Int J Food Microbiol 2008;124:91-7. |
|3.||Pascual ME, Slowing K, Carretero E, Mata DS, Villar A. Lippia: Traditional uses, chemistry and pharmacology: A review. J Ethnopharmacol 2001;76:201-14. |
|4.||Oliveira GP, Carvalhaes MA, Queiroz M Jr, Tien OS, Kakinami SH, et al. Medicinal plants popularly used in the Brazilian Tropical Atlantic Forest. Fitoterapia 2002;73:69-91. |
|5.||Lorenzi H, Matos FJ. Farmacologia. In: Plantas medicinais do Brasil: Nativas e Exóticas Cultivadas, Nova Odessa, São Paulo; 2002. p. 615. |
|6.||Vannuffel P, Cocito C. Mechanism of action of streptogramins and macrolides. Drugs 1996;51:20-30. |
|7.||Therapeutic Guidelines: Antibiotic. Version 12. Melbourne: Therapeutic Guidelines Ltd; 2002. |
|8.||Tavares DA, Sa-Leao R, Miragaia M, Large H. Screening of CA-MRSA among Staphylococcus aureus colonizing healthy young children living in two areas (urban and rural) of Portugal. BMC Infect Dis 2010;10:110. |
|9.||Alencar JW, Craveiro AA, Matos FJ, Machado MI. Kovats indices simulation in essential oils analysis. Quim Nova 1990;13:282-4. |
|10.||Adams RP. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. 4 th ed. Carol Stream, Illinois: Allured Publishing Corporation; 2007. |
|11.||Rodrigues FF, Costa JG, Coutinho HD. Synergy effects of the antibiotics gentamicin and the essential oil of Croton zehntneri. Phytomedicine 2009;16:1052-5. |
|12.||Schuck VJ, Fratini M, Rauber CS, Henriques A, Schapoval EE. Avaliação da atividade antimicrobiana de Cymbopogon citratus. Rev Bras Cien Farm 2001;37:45-9. |
|13.||Onawunmi GO. Evaluation of the antimicrobial activity of citral. Lett Appl Microbiol 1989;9:105-8. |
|14.||Vera JR, Pastrana PF, Fernández K, Viña A. Actividad antimicrobiana in vitro de volátiles y no volátiles de Lippia alba y extractos orgânicos y acuoso de Justicia pectoralis cultivadas em diferentes pisos térmicos del departamento Del Tolima. Sci Tech 2007;33:345-8. |
|15.||Aguiar JS, Costa MC, Nascimento SC, Sena KX. Atividade antimicrobiana de Lippia alba (Mill.) N. E. Brown (Verbenaceae). Rev Bras Farmacogn 2008;18:436-40. |
|16.||Ara N, Nur MH, Amran MS, Wahid MI, Ahmed M. In vitro antimicrobial and cytotoxic activities of leaves and flowers extracts from Lippia alba. Pak J Biol Sci 2009;12:87-90. |
|17.||Nicolson K, Evans G, Toole PW. Potentiation of methicillin activity against methicillin-resistant Staphylococcus aureus by diterpenes. FEMS Microbiol Lett 1999;179:233-9. |
|18.||Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82. |
|19.||Brehm-Stecher BF, Johnson EA. Sensitization of Staphylococcus aureus and Escherichia coli to antibiotics by the sesquiterpenoids nerolidol, farnesol, bisabolol, and apritone. Antimicrob Agents Chemother 2003;47:3357-60. |
|20.||Wendakoon CN, Sakaguchi M. Inhibition of amino acid decarboxylase activity of Enterobacter aerogenes by active components in spices. J Food Prot 1995;58:280-3. |
|21.||Sharafi SM, Rasooli I, Owlia P, Taghizadeh M, Astaneh SA. Protective effects of bioactive phytochemicals from Mentha piperita with multiple health potentials. Pharmacogn Mag 2010;6:147-53. |
|22.||Costa JM, Campos AR, Brito SA, Pereira CB, Souza EO, Rodrigues FG. Biological screening of Araripe basin medicinal plants using Artemia salina Leach and pathogenic bacteria. Pharmacogn Mag 2010;6:331-4. |
|23.||Simões CM, Schenkel EP, Gosmann G, Mello JC, Mentz LA, Petrovick PR. In: Farmacognosia: da planta ao medicamento. 4 th ed. Porto Alegre: UFSC; 2002. |
|24.||Veiga VF Jr. Estudo do consumo de plantas medicinais na Região Centro-Norte do Estado do Rio de Janeiro: Aceitação pelos profissionais de saúde e modo de uso pela população. Rev Bras Farmacogn 2008;18:308-13. |
|25.||Tôrres AR, Oliveira RA, Diniz MF, Araújo EC. Estudo sobre o uso de plantas medicinais em crianças hospitalizadas da cidade de João Pessoa: Riscos e benefícios. Rev Bras Farmacogn 2005;15:373-80. |
|26.||Oliveira RA, Lima EO, Vieira WL, Freire KR, Trajano VN, Lima IO, Souza EL, Toledo MS, Silva-Filho RN. Estudo da interferência de óleos essenciais sobre a atividade de alguns antibióticos usados na clínica. Rev Bras Farmacogn 2006;16:77-82. |
|27.||Hemaiswaryaa S, Kruthiventib AK, Doblea M. Synergism between natural products and antibiotics against infectious diseases. Phytomedicine 2008;15:639-52. |
|28.||Simões M, Rocha S, Coimbra MA, Vieira MJ. Enhancement of Escherichia coli and Staphylococcus aureus antibiotic susceptibility using sesquiterpenoids. Med Chem 2008;4:616-23. |
|29.||Sousa EO, Silva NF, Rodrigues FF, Campos AR, Lima SG, Costa JG. Chemical composition and resistance-modifying effect of the essential oil of Lantana camara Linn. Pharmacogn Mag 2010;6:79-82. |
[Table 1], [Table 2]
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