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ORIGINAL ARTICLE
Year : 2011  |  Volume : 7  |  Issue : 28  |  Page : 277-283

A high-performance molluscicidal ingredient against Oncomelania hupensis produced by a rhizospheric strain from Phytolacca acinosa Roxb


1 School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu; College of Life Science and Technology, Henan Institute of Science and Technology, Henan, China
2 School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu, China
3 School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu; Zhenjiang Center for Disease Control and Prevention, Zhenjiang, Jiangsu, China

Correspondence Address:
Jun Chen
School of Pharmacy, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu Province, 212013
China
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Source of Support: Research and Innovation Project for Postgraduate of Higher Education Institutions of Jiangsu Province in 2011 (Grant No.CXZZ11_0587), Conflict of Interest: None


DOI: 10.4103/0973-1296.90398

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Background: Snail (Oncomelania hupensis) control is an important and effective preventive strategy in schistosomiasis control programs, and screening microbial molluscicidal agents is one of the most promising categories in biomolluscicides. Objective: To purify and identify the molluscicidal ingredient (MI) obtained from strain SL-30's exocellular broth. Materials and Methods: The active extracts extracted from SL-30's exocellular broth was purified on a silica gel column guided by molluscicidal activity assay against Oncomelania hupensis, then the MI was obtained. NMR spectroscopy and LC-MS/MS analysis was used to identify the molecular structure of the MI. Results: Molluscicidal activity bioassay showed that the MI exhibited significant molluscicidal activity with the LC 50 values of 0.101, 0.062, and 0.022 mg/L, respectively, in the case of exposure period of 24 h. From 1 H NMR, 13 C NMR, 1 H- 1 H COSY, and 1 H- 13 C HSQC spectra, partial important structure fragment was obtained, and the relative molecular weight of the MI showed 326 according to LC-MS analysis. Then, on these grounds, it was indicated that the molecular structure of the MI had a higher similarity to Gliotoxin with the molecular formula of C 13 H 14 N 2 O 4 S 2 . The quasi-molecular ion of m/z 325.45 was further analyzed by MS 2 as the parent ion, and two daughter ions obtained at m/z 295.11 [M-CH 2 OH]- and m/z 261.08 [M-CH 2 OH -2S]−.Conclusion: The MI was finally confirmed as Gliotoxin.


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