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ORIGINAL ARTICLE
Year : 2011  |  Volume : 7  |  Issue : 27  |  Page : 186-192

Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze


1 Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng - 224002, People's Republic of China
2 Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng - 224002; School of Pharmacy, Jiangsu University, Zhenjiang - 212013, People's Republic of China
3 School of Pharmacy, Jiangsu University, Zhenjiang - 212013, People's Republic of China

Correspondence Address:
Jing Gao
School of Pharmacy, Jiangsu University, Zhenjiang, 212013
People's Republic of China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.84225

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Objective: To optimize the extraction technology for polysaccharides from the root of Limonium sinense (Girard) Kuntze, Plumbaginaceae and evaluate the antioxidant capacity of polysaccharides from L. sinense (LSEP) Materials and Methods : One-singer factor and response surface methodology(RSM) were established to extract the polysaccharides from L. sinense. Then, the 1,1-diphenyl-2-picrylhydrazyl free radical, hydroxyl radical(.OH), and 2,2'-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt free radical assays were established to measure the antioxidant capacity of the LSEP in vitro. Results: According to analysis, extraction temperature significantly affected extraction yield. The optimum extraction conditions for LSEP were as follows: extraction temperature, 95°C; ultrasonic time 50 minutes; and dosage liquor ratio, 1: 12. Under these conditions, the experimental yield of crude LSEP was 12.80±0.19% which was well matched with the predicted models. The antioxidant capacity data suggested that LSEP has strong antioxidant activity. Conclusion: One-singer factor and RSM were used to extract of LSEP are simple and feasible and LSEP could be developed as a nutraceutical agent for itsstrong antioxidant activity.


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