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ORIGINAL ARTICLE
Year : 2011  |  Volume : 7  |  Issue : 25  |  Page : 40-45

Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures


1 State Key Laboratory of Food Science & Technology, Nanchang University, Nanchang - 330 047, Jiangxi, China
2 Department of chemistry, Nanchang University, Nanchang - 330 031, Jiangxi, China
3 Jiangxi University of Traditional Chinese Medicine, Nanchang - 330 006, Jiangxi, China
4 Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi - 830 011, Xinjiang, China
5 State Key Laboratory of Food Science & Technology, Nanchang University, Nanchang - 330 047, Jiangxi; Department of chemistry, Nanchang University, Nanchang - 330 031, Jiangxi, China

Correspondence Address:
Shuwen Cao
State Key Laboratory of Food Science & Technology, Nanchang University, Nanchang - 330 047, Jiangxi
China
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Source of Support: This project was supported by the graduate student innovation fund of Jiangxi (No.YC10A019), Conflict of Interest: None


DOI: 10.4103/0973-1296.75900

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Background: Extraction temperature influences the total phenolic content (TPC), total flavonoid content (TFC) of medicinal plant extracts to a great extend. TPC and TFC are the principle activity constituents present in the plant. The effects of extraction temperature on TPC, TFC and free radical-scavenging capacity of Gynura divaricata leaf extracts are worth to study. Materials and Methods: Folin-Ciocalteu and aluminum chloride colorimetric assay were used to determine the TPC and TFC of Gynura divaricata leaf extracts at different temperatures. The antioxidant and free radical-scavenging activity were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and phosphomolybdenum methods. Results: TPC and TFC were significantly elevated with increasing extraction temperature (from 40°C to 100°C). However, TPC and TFC were not significantly different (P > 0.05) at the extraction temperatures 90°C and 100°C. Also, the extracts obtained at a higher temperature exhibited a significant free radical-scavenging activity compared with extraction at lower temperatures (P < 0.05). The TPCs (13.95-36.68 mg gallic acid equivalent/g dry material) were highly correlated with DPPH (R2 = 0.9229), ABTS (R2 = 0.9951) free radical-scavenging capacity, and total antioxidant activity (R2 = 0.9872) evaluated by phosphomolybdenum method. Conclusion: The TPC and TFC of G. divaricata leaf was significantly influenced by the extraction temperatures, which were the main antioxidant constituents present in the G. divaricata plant.


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