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ORIGINAL ARTICLE
Year : 2010  |  Volume : 6  |  Issue : 22  |  Page : 98-101

Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography


1 Xinjiang Key Laboratory of Famous Prescription and Science of Formulas; and College of Pharmacy, XinJiang Medical University, Urumqi - 830 011, China
2 Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Scotland-G1 1QE, United Kingdom
3 Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi - 830 011, China

Correspondence Address:
Shuge Tian
NO 393 Xinyi Road, Xinshi District Urumqi Xinjiang, Urumqi -830 011
China
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Source of Support: China, the Xinjiang Province Office of Science and Technology Funding (Grant NO: PT07-08), Conflict of Interest: None


DOI: 10.4103/0973-1296.62893

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A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C 18 4.6 mmΧ50 mm, 1.8 μm) column with mobile phase complosed of methanol and 0.05 mol/l potassium phosphate monobasic. Flow rate was at 1.0 ml/min. Retention time was at about 1.16 min. Recovery rate was 99.8% and relative standard deviation of three replicate samples tested was 1.8%. RRLC method has been applied to both in vitro studies of paeoniflorin formulation and drug estimation in biological samples.


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