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RESEARCH ARTICLE
Year : 2009  |  Volume : 5  |  Issue : 18  |  Page : 145-150

In Vitro Propagation of Desmodium gangeticum (L.) DC. from Cotyledonary Nodal Explants


Regional Research Institute (Ay.), Kothrud, Pune - 4110 38 (MH), India

Correspondence Address:
A M Gurav
Regional Research Institute (Ay.), Kothrud, Pune - 4110 38 (MH)
India
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Source of Support: None, Conflict of Interest: None


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An in vitro procedure for rapid multiplication of medicinally important plant Desmodium gangeticum (L.) DC. (Fabaceae), has been developed using cotyledonary nodal explant. An average of 9.2 shoots per explant were obtained by culturing cotyledonary nodal explaint on Murashige and Skoog's medium containing 8.8 μM BAP and 21.2 μM NAA, in combination, within 28 days. These shoots were rooted on half strength MS medium supplemented with IAA 17.1 μM. Rooted plantlets were hardened using 1:1:1 mixture of soil, river sand and vermiculite under green house conditions.


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